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Title   Cytochrome P450 3E1효소유전자 다형성분석을 통한 자궁겨부암의 유전적 감수성에 관한 연구 ( Cytochrome P450 2E1 polymorphisms and Genetic Susceptinilitu to Cervical Cancer )
Publicationinfo   1997 Jan; 029(03): 429-436.
Key_word   Cervical cancer, Genetic susceptibility, Cytochrome P450 2E1
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Abstract   Purpose: Interindividual genetic differences in susceptibility to chemical carcinogens are one of the most important host factors in human cancer. The genetically determined differences in metabolism, related to cytochrome P450 (CYP450) genes have been reported to be associated with various cancer susceptibility. The present study was set up to establish the frequency of the polymorphic genotypes of two CYP450 (CYP2E1/PstI and CYP2E1/DraI) isozymes in Korea, to evaluate a possible increased incidence of the genotype associated with higher cervical cancer risks among Korean cervical cancer patients. Materials and Methods: In this study, extracted DNAs from 228 cervical cancer patients and 360 normal healthy controls were analysed with the polymerase chain reaction-restriction fragment length polymosphism (PCR-RFLP) method. Results: In the CYP 2E1 genotypes, detected by PstI or RsaI digestion, there were no statistically remarkable differences between the cervical cancer patients and control groups. And when the cervical cancer patients were divided into subgroups with respect to the age, the frequency of CYP 2E1/PstI polymorphisms in the cervical cancer patients under the 40 years old was not significantly higher compared to the controls or the patients above the 40 years old and, c1/c1 genotype was prominent in this type of polymorphism. The frequency of CYP 2E1/DraI polymorphisms in the cervical cancer patients was not significantly higher compared to the controls, and D/D genotype was prominent in this type of polymorphism. In cervical carcinoma, the polymorphic genotypes of CYP 2E1 were not correlated to other parameters including clinical stage, histological tumor type, and degree of differentiation. Conclusion: These results suggest that individuals carrying CYP 2E1/PstI (c1/cl) or CYP 2E1/DraI (D/D) alleles are not genetically susceptible to cervical cancer in Korea.
저 자   김진우(Jin Woo Kim),이춘근(Choon Geun Lee),손여원(Ye Won Son),민홍기(Hong Ki Min),이경욱(Kyung Wook Lee),권녕주(Nyong Joo Kwon),고현숙(Hyun Sook Ko),신진웅(Jin Woong Shin),김흥기(Heung Ki Kim),김태응(Tae Eung Kim),이준모(Joon Mo Lee),남궁성은(Sung Eun NamGoong)